View source for 7wjf


==Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase mutant D394A in complex with kojibiose==
<StructureSection load='7wjf' size='340' side='right'caption='[[7wjf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris_MG1363 Lactococcus lactis subsp. cremoris MG1363]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wjf OCA], [https://pdbe.org/7wjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wjf RCSB], [https://www.ebi.ac.uk/pdbsum/7wjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wjf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A2RM80_LACLM A2RM80_LACLM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Carbohydrate-active enzymes (CAZymes) are involved in the degradation, biosynthesis, and modification of carbohydrates, and vary with the diversity of carbohydrates. The glycoside hydrolase (GH) family 31 is one of the most diverse families of CAZymes, containing various enzymes that act on alpha-glycosides. However, the function of some GH31 groups remains unknown, as their enzymatic activity is difficult to estimate due to the low amino acid sequence similarity between characterized and uncharacterized members. Here, we performed a phylogenetic analysis and discovered a protein cluster (GH31_u1) sharing low sequence similarity with the reported GH31 enzymes. Within this cluster, we showed that a GH31_u1 protein from Lactococcus lactis (LlGH31_u1) and its fungal homolog demonstrated hydrolytic activities against nigerose [alpha-D-Glcp-(1--&gt;3)-D-Glc]. The kcat/Km values of LlGH31_u1 against kojibiose and maltose were 13% and 2.1% of that against nigerose, indicating that LlGH31_u1 has a higher specificity to the alpha-1,3 linkage of nigerose than other characterized GH31 enzymes, including eukaryotic enzymes. Furthermore, the three-dimensional structures of LlGH31_u1 determined using X-ray crystallography and cryogenic electron microscopy revealed that LlGH31_u1 forms a hexamer and has a C-terminal domain comprising four alpha-helices, suggesting that it contributes to hexamerization. Finally, crystal structures in complex with nigerooligosaccharides and kojibiose along with mutational analysis revealed the active site residues involved in substrate recognition in this enzyme. This study reports the first structure of a bacterial GH31 alpha-1,3-glucosidase and provides new insight into the substrate specificity of GH31 enzymes and the physiological functions of bacterial and fungal GH31_u1 members.

Structural basis of the strict specificity of a bacterial GH31 alpha-1,3-glucosidase for nigerooligosaccharides.,Ikegaya M, Moriya T, Adachi N, Kawasaki M, Park EY, Miyazaki T J Biol Chem. 2022 Mar 12:101827. doi: 10.1016/j.jbc.2022.101827. PMID:35293315<ref>PMID:35293315</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7wjf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Xylosidase 3D structures|Xylosidase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Lactococcus lactis subsp. cremoris MG1363]]
[[Category: Large Structures]]
[[Category: Ikegaya M]]
[[Category: Miyazaki T]]