View source for 7wkq


==Crystal Structure of halohydrin dehalogenase from Acidimicrobiia bacterium==
<StructureSection load='7wkq' size='340' side='right'caption='[[7wkq]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wkq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidimicrobiia_bacterium Acidimicrobiia bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WKQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wkq OCA], [https://pdbe.org/7wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wkq RCSB], [https://www.ebi.ac.uk/pdbsum/7wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wkq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2G4FD71_9ACTN A0A2G4FD71_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report the discovery of an unusual halohydrin dehalogenase, HHDHamb, that can work under relative low acidic conditions and extremely low temperatures for the bio-nitration of epoxides using nitrite as a nitrating agent. The bio-nitration strategy exhibits high chemo-, regio-, and enantioselectivity, catalyzing the kinetic resolution of various epoxides to enantiopure beta -nitroalcohols with nitro-bearing stereocenters in up to 41% isolated yield and &gt;99% enantiomeric excess ( ee ). Additionally, the bio-nitration method displays a high reaction efficiency and can be performed on a gram scale. We also solved the crystal structure of HHDHamb to understand the possible structural determinants of chemoselectivity control in the bio-nitration reaction.

Identification and Structure Analysis of an Unusual Halohydrin Dehalogenase for Highly Chemo-, Regio- and Enantioselective Bio-Nitration of Epoxides.,Wang HH, Wan NW, Miao RP, He CL, Chen YZ, Liu ZQ, Zheng YG Angew Chem Int Ed Engl. 2022 Jul 20. doi: 10.1002/anie.202205790. PMID:35856897<ref>PMID:35856897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7wkq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acidimicrobiia bacterium]]
[[Category: Large Structures]]
[[Category: Wan NW]]