View source for 7wlv


==Crystal Structure of the Multidrug effulx transporter BpeF from Burkholderia pseudomallei.==
<StructureSection load='7wlv' size='340' side='right'caption='[[7wlv]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wlv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WLV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wlv OCA], [https://pdbe.org/7wlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wlv RCSB], [https://www.ebi.ac.uk/pdbsum/7wlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wlv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q63NK6_BURPS Q63NK6_BURPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BpeB and BpeF are multidrug efflux transporters from Burkholderia pseudomallei that enable multidrug resistance. Here, we report the crystal structures of BpeB and BpeF at 2.94 A and 3.0 A resolution, respectively. BpeB was found as an asymmetric trimer, consistent with the widely-accepted functional rotation mechanism for this type of transporter. One of the monomers has a distinct structure that we interpret as an intermediate along this functional cycle. Additionally, a detergent molecule bound in a previously undescribed binding site provides insights into substrate translocation through the pathway. BpeF shares structural similarities with the crystal structure of OqxB from Klebsiella pneumoniae, where both are symmetric trimers composed of three "binding"-state monomers. The structures of BpeB and BpeF further our understanding of the functional mechanisms of transporters belonging to the HAE1-RND superfamily.

Crystal structures of multidrug efflux transporters from Burkholderia pseudomallei suggest details of transport mechanism.,Kato T, Okada U, Hung LW, Yamashita E, Kim HB, Kim CY, Terwilliger TC, Schweizer HP, Murakami S Proc Natl Acad Sci U S A. 2023 Jul 18;120(29):e2215072120. doi: , 10.1073/pnas.2215072120. Epub 2023 Jul 10. PMID:37428905<ref>PMID:37428905</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7wlv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Burkholderia pseudomallei K96243]]
[[Category: Large Structures]]
[[Category: Hung L-W]]
[[Category: Kato T]]
[[Category: Murakami S]]
[[Category: Okada U]]
[[Category: Terwilliger TC]]
[[Category: Yamashita E]]