View source for 7woi


==Structure of the shaft pilin Spa2 from Corynebacterium glutamicum==
<StructureSection load='7woi' size='340' side='right'caption='[[7woi]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7woi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_14067 Corynebacterium glutamicum ATCC 14067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WOI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7woi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7woi OCA], [https://pdbe.org/7woi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7woi RCSB], [https://www.ebi.ac.uk/pdbsum/7woi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7woi ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteria can be programmed to create engineered living materials (ELMs) with self-healing and evolvable functionalities. However, further development of ELMs is greatly hampered by the lack of engineerable nonpathogenic chassis and corresponding programmable endogenous biopolymers. Here, we describe a technological workflow for facilitating ELMs design by rationally integrating bioinformatics, structural biology and synthetic biology technologies. We first develop bioinformatics software, termed Bacteria Biopolymer Sniffer (BBSniffer), that allows fast mining of biopolymers and biopolymer-producing bacteria of interest. As a proof-of-principle study, using existing pathogenic pilus as input, we identify the covalently linked pili (CLP) biosynthetic gene cluster in the industrial workhorse Corynebacterium glutamicum. Genetic manipulation and structural characterization reveal the molecular mechanism of the CLP assembly, ultimately enabling a type of programmable pili for ELM design. Finally, engineering of the CLP-enabled living materials transforms cellulosic biomass into lycopene by coupling the extracellular and intracellular bioconversion ability.

Accelerating the design of pili-enabled living materials using an integrative technological workflow.,Huang Y, Wu Y, Hu H, Tong B, Wang J, Zhang S, Wang Y, Zhang J, Yin Y, Dai S, Zhao W, An B, Pu J, Wang Y, Peng C, Li N, Zhou J, Tan Y, Zhong C Nat Chem Biol. 2024 Feb;20(2):201-210. doi: 10.1038/s41589-023-01489-x. Epub 2023 , Nov 27. PMID:38012344<ref>PMID:38012344</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7woi" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pilin 3D structures|Pilin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Corynebacterium glutamicum ATCC 14067]]
[[Category: Large Structures]]
[[Category: Huang YY]]
[[Category: Wang LT]]
[[Category: Wu YF]]
[[Category: Zhong C]]
[[Category: Zhou J]]