View source for 7wxu


==GPR110/Gq complex==
<StructureSection load='7wxu' size='340' side='right'caption='[[7wxu]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wxu]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WXU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wxu OCA], [https://pdbe.org/7wxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wxu RCSB], [https://www.ebi.ac.uk/pdbsum/7wxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wxu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Adhesion G protein-coupled receptors (aGPCRs) are keys of many physiological events and attractive targets for various diseases. aGPCRs are also known to be capable of self-activation via an autoproteolysis process that removes the inhibitory GAIN domain on the extracellular side of receptor and releases a stalk peptide to bind and activate the transmembrane side of receptor. However, the detailed mechanism of aGPCR activation remains elusive. Here, we report the cryo-electron microscopy structures of GPR110 (ADGRF1), a member of aGPCR, in complex with G(q), G(s), G(i), G(12) and G(13.) The structures reveal distinctive ligand engaging model and activation conformations of GPR110. The structures also unveil the rarely explored GPCR/G(12) and GPCR/G(13) engagements. A comparison of G(q), G(s), G(i), G(12) and G(13) engagements with GPR110 reveals details of G-protein engagement, including a dividing point at the far end of the alpha helix 5 (alphaH5) of Galpha subunit that separates G(q)/G(s) engagements from G(i)/G(12)/G(13) engagements. This is also where G(q)/G(s) bind the receptor through both hydrophobic and polar interaction, while G(i)/G(12)/G(13) engage receptor mainly through hydrophobic interaction. We further provide physiological evidence of GPR110 activation via stalk peptide. Taken together, our study fills the missing information of GPCR/G-protein engagement and provides a framework for understanding aGPCR activation and GPR110 signaling.

Structural basis of adhesion GPCR GPR110 activation by stalk peptide and G-proteins coupling.,Zhu X, Qian Y, Li X, Xu Z, Xia R, Wang N, Liang J, Yin H, Zhang A, Guo C, Wang G, He Y Nat Commun. 2022 Sep 20;13(1):5513. doi: 10.1038/s41467-022-33173-4. PMID:36127364<ref>PMID:36127364</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7wxu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transducin 3D structures|Transducin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: He Y]]
[[Category: Zhu X]]