View source for 7x14


==Crystal structure of phospho-FFAT motif of MIGA2 bound to VAPB==
<StructureSection load='7x14' size='340' side='right'caption='[[7x14]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7x14]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculoides Mus musculoides] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X14 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.675&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x14 OCA], [https://pdbe.org/7x14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x14 RCSB], [https://www.ebi.ac.uk/pdbsum/7x14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x14 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VAPB_MOUSE VAPB_MOUSE] Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.[UniProtKB:O95292]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The endoplasmic reticulum (ER)-mitochondria contact site (ERMCS) is crucial for exchanging biological molecules such as phospholipids and Ca(2+) ions between these organelles. Mitoguardin-2 (MIGA2), a mitochondrial outer membrane protein, forms the ERMCS in higher eukaryotic cells. Here, we report the crystal structures of the MIGA2 Lipid Droplet (LD) targeting domain and the ER membrane protein VAPB bound to the phosphorylated FFAT motif of MIGA2. These structures reveal that the MIGA2 LD targeting domain has a large internal hydrophobic pocket that accommodates phospholipids and that two phosphorylations of the FFAT motif are required for tight interaction of MIGA2 with VAPB, which enhances the rate of lipid transport. Further biochemical studies show that MIGA2 transports phospholipids between membranes with a strong preference for binding and trafficking phosphatidylserine (PS). These results provide a structural and molecular basis for understanding how MIGA2 mediates the formation of ERMCS and facilitates lipid trafficking at the ERMCS.

Structural basis for mitoguardin-2 mediated lipid transport at ER-mitochondrial membrane contact sites.,Kim H, Lee S, Jun Y, Lee C Nat Commun. 2022 Jun 28;13(1):3702. doi: 10.1038/s41467-022-31462-6. PMID:35764626<ref>PMID:35764626</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7x14" style="background-color:#fffaf0;"></div>

==See Also==
*[[Vesicle-associated membrane protein 3D structures|Vesicle-associated membrane protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculoides]]
[[Category: Mus musculus]]
[[Category: Kim H]]
[[Category: Lee C]]