View source for 7xa7


==Crystal structure of SARS-CoV-2 receptor-binding domain in complex with intermediate horseshoe bat ACE2==
<StructureSection load='7xa7' size='340' side='right'caption='[[7xa7]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xa7]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhinolophus_affinis Rhinolophus affinis] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XA7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.31&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xa7 OCA], [https://pdbe.org/7xa7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xa7 RCSB], [https://www.ebi.ac.uk/pdbsum/7xa7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xa7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A7D7FA76_RHIAI A0A7D7FA76_RHIAI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused a global pandemic. Intermediate horseshoe bats (Rhinolophus affinis) are hosts of RaTG13, the second most phylogenetically related viruses to SARS-CoV-2. We report the binding between intermediate horseshoe bat ACE2 (bACE2-Ra) and SARS-CoV-2 receptor-binding domain (RBD), supporting the pseudotyped SARS-CoV-2 viral infection. A 3.3 A resolution crystal structure of the bACE2-Ra/SARS-CoV-2 RBD complex was determined. The interaction networks of Patch 1 showed differences in R34 and E35 of bACE2-Ra compared to hACE2 and big-eared horseshoe bat ACE2 (bACE2-Rm). The E35K substitution, existing in other species, significantly enhanced the binding affinity owing to its electrostatic attraction with E484 of SARS-CoV-2 RBD. Furthermore, bACE2-Ra showed extensive support for the SARS-CoV-2 variants. These results broaden our knowledge of the ACE2/RBD interaction mechanism and emphasize the importance of continued surveillance of intermediate horseshoe bats to prevent spillover risk.

Structural basis of SARS-CoV-2 and its variants binding to intermediate horseshoe bat ACE2.,Tang L, Zhang D, Han P, Kang X, Zheng A, Xu Z, Zhao X, Wang VY, Qi J, Wang Q, Liu K, Gao GF Int J Biol Sci. 2022 Jul 11;18(12):4658-4668. doi: 10.7150/ijbs.73640. , eCollection 2022. PMID:35874946<ref>PMID:35874946</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xa7" style="background-color:#fffaf0;"></div>

==See Also==
*[[Spike protein 3D structures|Spike protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhinolophus affinis]]
[[Category: Severe acute respiratory syndrome coronavirus 2]]
[[Category: Han P]]
[[Category: Qi JX]]
[[Category: Tang LF]]
[[Category: Zhang D]]