View source for 7xbs


==Crystal structure of the adenylation domain of CmnG==
<StructureSection load='7xbs' size='340' side='right'caption='[[7xbs]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xbs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharothrix_mutabilis_subsp._capreolus Saccharothrix mutabilis subsp. capreolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XBS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xbs OCA], [https://pdbe.org/7xbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xbs RCSB], [https://www.ebi.ac.uk/pdbsum/7xbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xbs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A6YEH8_STRMP A6YEH8_STRMP] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Capreomycidine (Cap) is a nonproteinogenic amino acid and building block of nonribosomal peptide (NRP) natural products. We report the formation and activation of Cap in capreomycin biosynthesis. CmnC and CmnD catalyzed hydroxylation and cyclization, respectively, of l-Arg to form l-Cap. l-Cap is then adenylated by CmnG-A before being incorporated into the nonribosomal peptide. The co-crystal structures of CmnG-A with l-Cap and adenosine nucleotides provide insights into the specificity and engineering opportunities of this unique adenylation domain.

Characterization and Structural Determination of CmnG-A, the Adenylation Domain That Activates the Nonproteinogenic Amino Acid Capreomycidine in Capreomycin Biosynthesis.,Chen IH, Cheng T, Wang YL, Huang SJ, Hsiao YH, Lai YT, Toh SI, Chu J, Rudolf JD, Chang CY Chembiochem. 2022 Dec 16;23(24):e202200563. doi: 10.1002/cbic.202200563. Epub , 2022 Nov 16. PMID:36278314<ref>PMID:36278314</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xbs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharothrix mutabilis subsp. capreolus]]
[[Category: Chang CY]]
[[Category: Chen IH]]
[[Category: Wang YL]]