View source for 7xhn


==Structure of human inner kinetochore CCAN-DNA complex==
<StructureSection load='7xhn' size='340' side='right'caption='[[7xhn]], [[Resolution|resolution]] 3.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xhn]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.71&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhn OCA], [https://pdbe.org/7xhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhn RCSB], [https://www.ebi.ac.uk/pdbsum/7xhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CENPO_HUMAN CENPO_HUMAN] Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex.<ref>PMID:16622420</ref> <ref>PMID:16716197</ref> <ref>PMID:16932742</ref> <ref>PMID:18007590</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In mitosis, accurate chromosome segregation depends on kinetochores that connect centromeric chromatin to spindle microtubules. The centromeres of budding yeast, which are relatively simple, are connected to individual microtubules via a kinetochore constitutive centromere associated network (CCAN). However, the complex centromeres of human chromosomes comprise millions of DNA base pairs and attach to multiple microtubules. Here, by use of cryo-electron microscopy and functional analyses, we reveal the molecular basis of how human CCAN interacts with duplex DNA and facilitates accurate chromosome segregation. The overall structure relates to the cooperative interactions and interdependency of the constituent sub-complexes of the CCAN. The duplex DNA is topologically entrapped by human CCAN. Further, CENP-N does not bind to the RG-loop of CENP-A but to DNA in the CCAN complex. The DNA binding activity is essential for CENP-LN localization to centromere and chromosome segregation during mitosis. Thus, these analyses provide new insights into mechanisms of action underlying kinetochore assembly and function in mitosis.

Structural insights into human CCAN complex assembled onto DNA.,Tian T, Chen L, Dou Z, Yang Z, Gao X, Yuan X, Wang C, Liu R, Shen Z, Gui P, Teng M, Meng X, Hill DL, Li L, Zhang X, Liu X, Sun L, Zang J, Yao X Cell Discov. 2022 Sep 9;8(1):90. doi: 10.1038/s41421-022-00439-6. PMID:36085283<ref>PMID:36085283</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xhn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Sun LF]]
[[Category: Tian T]]
[[Category: Wang CL]]
[[Category: Yang ZS]]
[[Category: Zang JY]]