View source for 7xhs


==Crystal structure of CipA crystal produced by cell-free protein synthesis==
<StructureSection load='7xhs' size='340' side='right'caption='[[7xhs]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xhs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus Photorhabdus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XHS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xhs OCA], [https://pdbe.org/7xhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xhs RCSB], [https://www.ebi.ac.uk/pdbsum/7xhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xhs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7N6H4_PHOLL Q7N6H4_PHOLL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In-cell protein crystallization (ICPC) has been investigated as a technique to support the advancement of structural biology because it does not require protein purification and a complicated crystallization process. However, only a few protein structures have been reported because these crystals formed incidentally in living cells and are insufficient in size and quality for structure analysis. Here, we have developed a cell-free protein crystallization (CFPC) method, which involves direct protein crystallization using cell-free protein synthesis. We have succeeded in crystallization and structure determination of nano-sized polyhedra crystal (PhC) at a high resolution of 1.80 A. Furthermore, nanocrystals were synthesized at a reaction scale of only 20 muL using the dialysis method, enabling structural analysis at a resolution of 1.95 A. To further demonstrate the potential of CFPC, we attempted to determine the structure of crystalline inclusion protein A (CipA), whose structure had not yet been determined. We added chemical reagents as a twinning inhibitor to the CFPC solution, which enabled us to determine the structure of CipA at 2.11 A resolution. This technology greatly expands the high-throughput structure determination method of unstable, low-yield, fusion, and substrate-biding proteins that have been difficult to analyze with conventional methods.

Cell-free protein crystallization for nanocrystal structure determination.,Abe S, Tanaka J, Kojima M, Kanamaru S, Hirata K, Yamashita K, Kobayashi A, Ueno T Sci Rep. 2022 Oct 3;12(1):16031. doi: 10.1038/s41598-022-19681-9. PMID:36192567<ref>PMID:36192567</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xhs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photorhabdus]]
[[Category: Abe S]]
[[Category: Hirata K]]
[[Category: Kanamaru S]]
[[Category: Kojima M]]
[[Category: Tanaka J]]
[[Category: Ueno T]]
[[Category: Yamashita K]]