View source for 7xpk


==crystal structure of rice ASI1 BAH domain in complex with a rice SUVH6 peptide==
<StructureSection load='7xpk' size='340' side='right'caption='[[7xpk]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xpk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XPK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xpk OCA], [https://pdbe.org/7xpk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xpk RCSB], [https://www.ebi.ac.uk/pdbsum/7xpk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xpk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B9EY07_ORYSJ B9EY07_ORYSJ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dimethylated histone H3 Lys9 (H3K9me2) is a conserved heterochromatic mark catalyzed by SUPPRESSOR OF VARIEGATION 3-9 HOMOLOG (SUVH) methyltransferases in plants. However, the mechanism underlying the locus specificity of SUVH enzymes has long been elusive. Here, we show that a conserved N-terminal motif is essential for SUVH6-mediated H3K9me2 deposition in planta. The SUVH6 N-terminal peptide can be recognized by the bromo-adjacent homology (BAH) domain of the RNA- and chromatin-binding protein ANTI-SILENCING 1 (ASI1), which has been shown to function in a complex to confer gene expression regulation. Structural data indicate that a classic aromatic cage of ASI1-BAH domain specifically recognizes an arginine residue of SUVH6 through extensive hydrogen bonding interactions. A classic aromatic cage of ASI1 specifically recognizes an arginine residue of SUVH6 through extensive cation-pi interactions, playing a key role in recognition. The SUVH6-ASI1 module confers locus-specific H3K9me2 deposition at most SUVH6 target loci and gives rise to distinct regulation of gene expression depending on the target loci, either conferring transcriptional silencing or posttranscriptional processing of mRNA. More importantly, such mechanism is conserved in multiple plant species, indicating a coordinated evolutionary process between SUVH6 and ASI1. In summary, our findings uncover a conserved mechanism for the locus specificity of H3K9 methylation in planta. These findings provide mechanistic insights into the delicate regulation of H3K9 methylation homeostasis in plants.

Molecular basis of locus-specific H3K9 methylation catalyzed by SUVH6 in plants.,Zhang J, Yuan J, Lin J, Chen L, You LY, Chen S, Peng L, Wang CH, Du J, Duan CG Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2208525120. doi: , 10.1073/pnas.2211155120. Epub 2022 Dec 29. PMID:36580600<ref>PMID:36580600</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xpk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Du J]]
[[Category: Yuan J]]