View source for 7xsi


==SdnG, a Diels Alderase catalyzed the formation of norbornene skeleton in Sordarin biosynthetic pathway==
<StructureSection load='7xsi' size='340' side='right'caption='[[7xsi]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xsi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Sordaria_araneosa Sordaria araneosa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7xfk 7xfk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsi OCA], [https://pdbe.org/7xsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsi RCSB], [https://www.ebi.ac.uk/pdbsum/7xsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SDNG_SORAA SDNG_SORAA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sordarin (1) is a fungal diterpene glycoside that displays potent antifungal bioactivity through inhibition of elongation factor 2. The structures of sordarin and related compounds feature a highly rearranged tetracyclic diterpene core. In this study, we identified a concise pathway in the biosynthesis of sordarin. A diterpene cyclase (SdnA) generates the 5/8/5 cycloaraneosene framework, which is decorated by a set of P450s that catalyze a series of oxidation reactions, including hydroxylation, desaturation, and C-C bond oxidative cleavage, to give a carboxylate intermediate with a terminal alkene and a cyclopentadiene moiety. A novel Diels-Alderase SdnG catalyzes an intramolecular Diels-Alder (IMDA) reaction on this intermediate to forge the sordarin core structure. Subsequent methyl hydroxylation and glycosylation complete the biosynthesis of sordarin. Our work discloses a new strategy used by nature for the formation of the rearranged diterpene skeleton.

Biosynthesis of Sordarin Revealing a Diels-Alderase for the Formation of the Norbornene Skeleton.,Liu SH, Sun JL, Hu YL, Zhang L, Zhang X, Yan ZY, Guo X, Guo ZK, Jiao RH, Zhang B, Tan RX, Ge HM Angew Chem Int Ed Engl. 2022 Aug 15;61(33):e202205577. doi: , 10.1002/anie.202205577. Epub 2022 Jul 7. PMID:35701881<ref>PMID:35701881</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xsi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Sordaria araneosa]]
[[Category: Ge HM]]
[[Category: Zhang B]]