View source for 7xy8


==Crystal structure of antibody Fab fragment in complex with CD147(EMMPIRIN)==
<StructureSection load='7xy8' size='340' side='right'caption='[[7xy8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xy8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XY8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xy8 OCA], [https://pdbe.org/7xy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xy8 RCSB], [https://www.ebi.ac.uk/pdbsum/7xy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xy8 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CD147 is an immunoglobulin-like receptor that is highly expressed in various cancers and involved in the growth, metastasis, and activation of inflammatory pathways via interactions with various functional molecules, such as integrins, CD44, and monocarboxylate transporters. Through screening of CD147-targeting antibodies with antitumor efficacy, we discovered a novel rat monoclonal antibody (#)147D. This humanized IgG4-formatted antibody, h4(#)147D, showed potent antitumor efficacy in xenograft mouse models harboring the human PDAC cell line MIA PaCa-2, HCC cell line Hep G2, and CML cell line KU812, which featured low sensitivity to the corresponding standard-of-care drugs (gemcitabine, sorafenib, and imatinib, respectively). An analysis of tumor cells derived from MIA PaCa-2 xenograft mice treated with h4(#)147D revealed that cell surface expression of CD147 and its binding partners, including CD44 and integrin alpha3beta1/alpha6beta1, was significantly reduced by h4(#)147D. Inhibition of focal adhesion kinase (FAK), activation of multiple stress responsible signal proteins such as c-JunN-terminal kinase (JNK) and mitogen-activated protein kinase p38 (p38MAPK), and expression of SMAD4, as well as activation of caspase-3 were obviously observed in the tumor cells, suggesting that h4(#)147D induced tumor shrinkage by inducing multiple stress responsible signals. These results suggest that the anti-CD147 antibody h4(#)147D offers promise as a new antibody drug candidate.

Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals.,Fukuchi K, Nanai K, Yuita H, Maru C, Tsukada J, Ishigami M, Nagai Y, Nakano Y, Yoshimura C, Yoneda K, Amano M, Nakamura K, Oda Y, Nishigohri H, Yamamoto S, Ohnishi-Totoki Y, Inaki K, Komori H, Nakano R, Kanari Y, Nishida A, Matsui Y, Funo S, Takahashi S, Ohtsuka T, Agatsuma T J Oncol. 2022 Nov 4;2022:3552793. doi: 10.1155/2022/3552793. eCollection 2022. PMID:36385956<ref>PMID:36385956</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7xy8" style="background-color:#fffaf0;"></div>

==See Also==
*[[3D structures of basigin|3D structures of basigin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Amano M]]
[[Category: Fukuchi K]]
[[Category: Nakamura K]]
[[Category: Suzuki M]]
[[Category: Yoneda K]]