View source for 7y88


==class I diterpene synthase (CyS-GGPP-Mg2+) from Streptomyces cattleya==
<StructureSection load='7y88' size='340' side='right'caption='[[7y88]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7y88]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y88 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y88 OCA], [https://pdbe.org/7y88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y88 RCSB], [https://www.ebi.ac.uk/pdbsum/7y88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y88 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F8JK18_STREN F8JK18_STREN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS(C59A) variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes.

Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.,Xing B, Xu H, Li A, Lou T, Xu M, Wang K, Xu Z, Dickschat JS, Yang D, Ma M Angew Chem Int Ed Engl. 2022 Sep 5;61(36):e202209785. doi: , 10.1002/anie.202209785. Epub 2022 Aug 1. PMID:35819825<ref>PMID:35819825</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7y88" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces cattleya]]
[[Category: Ma M]]
[[Category: Xing B]]