View source for 7ycs


==Heterotetramer of Antitoxin PrpA together with Toxin PrpT from Pseudoalteromonas rubra==
<StructureSection load='7ycs' size='340' side='right'caption='[[7ycs]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ycs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_rubra Pseudoalteromonas rubra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YCS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ycs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ycs OCA], [https://pdbe.org/7ycs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ycs RCSB], [https://www.ebi.ac.uk/pdbsum/7ycs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ycs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0U2Y7P6_9GAMM A0A0U2Y7P6_9GAMM] 
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== Publication Abstract from PubMed ==
Bacteria could survive stresses by a poorly understood mechanism that contributes to the emergence of bacterial persisters exhibiting multidrug tolerance (MDT). Recently, Pseudoalteromonas rubra prpAT module was found to encode a toxin PrpT and corresponding cognate antidote PrpA. In this study, we first reported multiple individual and complex structures of PrpA and PrpT, which uncovered the high-resolution three-dimensional structure of the PrpT:PrpA(2):PrpT heterotetramer with the aid of size exclusion chromatography-multi-angle light scattering experiments (SEC-MALS). PrpT:PrpA(2):PrpT is composed of a PrpA homodimer and two PrpT monomers which are relatively isolated from each other and from ParE family. The superposition of antitoxin monomer structures from these structures highlighted the flexible C-terminal domain (CTD). A striking conformational change in the CTDs of PrpA homodimer depolymerized from homotetramer was provoked upon PrpT binding, which accounts for the unique PrpT-PrpA(RHH) mutual interactions and further neutralizes the toxin PrpT. PrpA(2-54)-form I and II crystal structures both contain a doughnut-shaped hexadecamer formed by eight homodimers organized in a cogwheel-like form via inter-dimer interface dominated by salt bridges and hydrogen bonds. Moreover, PrpA tends to exist in solution as a homodimer other than a homotetramer (SEC-MALS) in the absence of flexible CTD. Multiple multi-dimers, tetramer and hexamer included, of PrpA(2-54) mediated by the symmetric homodimer interface and the complicated inter-dimer interface could be observed in the solution. SEC-MALS assays highlighted that phosphate buffer (PB) and the increase in the concentration appear to be favorable for the PrpA(2-54) oligomerization in the solution. Taken together with previous research, a model of PrpA(2-54) homotetramer in complex with prpAT promoter and the improved mechanism underlying how PrpTA controls the plasmid replication were proposed here.

Structural insights into the PrpTA toxin-antitoxin system in Pseudoalteromonas rubra.,Wang C, Niu C, Hidayatullah KM, Xue L, Zhu Z, Niu L Front Microbiol. 2022 Nov 24;13:1053255. doi: 10.3389/fmicb.2022.1053255. , eCollection 2022. PMID:36504814<ref>PMID:36504814</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudoalteromonas rubra]]
[[Category: Niu LW]]
[[Category: Wang CC]]