View source for 7yfw


==Cyanophage Pam3 fiber==
<StructureSection load='7yfw' size='340' side='right'caption='[[7yfw]], [[Resolution|resolution]] 3.96&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yfw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_cyanophage Uncultured cyanophage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YFW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.96&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yfw OCA], [https://pdbe.org/7yfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yfw RCSB], [https://www.ebi.ac.uk/pdbsum/7yfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yfw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A9E7DT93_9CAUD A0A9E7DT93_9CAUD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater Myoviridae cyanophage Pam3, which has an icosahedral capsid of ~680 A in diameter, connected via a three-section neck to an 840-A-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology.

Fine structure and assembly pattern of a minimal myophage Pam3.,Yang F, Jiang YL, Zhang JT, Zhu J, Du K, Yu RC, Wei ZL, Kong WW, Cui N, Li WF, Chen Y, Li Q, Zhou CZ Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2213727120. doi: , 10.1073/pnas.2213727120. Epub 2023 Jan 19. PMID:36656854<ref>PMID:36656854</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7yfw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Uncultured cyanophage]]
[[Category: Jiang YL]]
[[Category: Yang F]]
[[Category: Zhou CZ]]