View source for 7yga


==Cryo-EM structure of Tetrahymena ribozyme conformation 2 undergoing the second-step self-splicing==
<StructureSection load='7yga' size='340' side='right'caption='[[7yga]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yga]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YGA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSU:URIDINE-5-PHOSPHOROTHIOATE'>SSU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yga OCA], [https://pdbe.org/7yga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yga RCSB], [https://www.ebi.ac.uk/pdbsum/7yga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yga ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Group I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing.

Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.,Li S, Palo MZ, Zhang X, Pintilie G, Zhang K Nat Commun. 2023 Mar 16;14(1):1294. doi: 10.1038/s41467-023-36724-5. PMID:36928031<ref>PMID:36928031</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7yga" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tetrahymena]]
[[Category: Greg P]]
[[Category: Li S]]
[[Category: Michael ZP]]
[[Category: Zhang K]]
[[Category: Zhang X]]