View source for 7ygw


==Crystal structure of the Zn2+-bound EFhd1/Swiprosin-2==
<StructureSection load='7ygw' size='340' side='right'caption='[[7ygw]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ygw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YGW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ygw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ygw OCA], [https://pdbe.org/7ygw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ygw RCSB], [https://www.ebi.ac.uk/pdbsum/7ygw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ygw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EFHD1_MOUSE EFHD1_MOUSE] Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (By similarity). May play a role in neuronal differentiation (PubMed:16336229).[UniProtKB:Q9BUP0]<ref>PMID:16336229</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
EF-hand proteins, which contain a Ca(2+)-binding EF-hand motif, are involved in regulating diverse cellular functions. Ca(2+) binding induces conformational changes that modulate the activities of EF-hand proteins. Moreover, these proteins occasionally modify their activities by coordinating metals other than Ca(2+), including Mg(2+), Pb(2+) and Zn(2+), within their EF-hands. EFhd1 and EFhd2 are homologous EF-hand proteins with similar structures. Although separately localized within cells, both are actin-binding proteins that modulate F-actin rearrangement through Ca(2+)-independent actin-binding and Ca(2+)-dependent actin-bundling activity. Although Ca(2+) is known to affect the activities of EFhd1 and EFhd2, it is not known whether their actin-related activities are affected by other metals. Here, the crystal structures of the EFhd1 and EFhd2 core domains coordinating Zn(2+) ions within their EF-hands are reported. The presence of Zn(2+) within EFhd1 and EFhd2 was confirmed by analyzing anomalous signals and the difference between anomalous signals using data collected at the peak positions as well as low-energy remote positions at the Zn K-edge. EFhd1 and EFhd2 were also found to exhibit Zn(2+)-independent actin-binding and Zn(2+)-dependent actin-bundling activity. This suggests the actin-related activities of EFhd1 and EFhd2 could be regulated by Zn(2+) as well as Ca(2+).

Structural and biochemical insights into Zn(2+)-bound EF-hand proteins, EFhd1 and EFhd2.,Mun SA, Park J, Kang JY, Park T, Jin M, Yang J, Eom SH IUCrJ. 2023 Mar 1;10(Pt 2):233-245. doi: 10.1107/S2052252523001501. PMID:36862489<ref>PMID:36862489</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ygw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Eom SH]]
[[Category: Jin M]]
[[Category: Kang JY]]
[[Category: Mun SA]]
[[Category: Park J]]
[[Category: Park T]]
[[Category: Yang J]]