View source for 7yim


==Cryo-EM structure of human Alpha-fetoprotein==
<StructureSection load='7yim' size='340' side='right'caption='[[7yim]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yim]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YIM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yim OCA], [https://pdbe.org/7yim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yim RCSB], [https://www.ebi.ac.uk/pdbsum/7yim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yim ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FETA_HUMAN FETA_HUMAN] Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
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== Publication Abstract from PubMed ==
Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, the homogeneity of ice thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and has become one of the main challenges for high-resolution cryo-EM. Here we found that the uniformity of thin ice relies on the surface flatness of the supporting film, and developed a method to use ultraflat graphene (UFG) as the support for cryo-EM specimen preparation to achieve better control of vitreous ice thickness. We show that the uniform thin ice on UFG improves the image quality of vitrified specimens. Using such a method we successfully determined the three-dimensional structures of hemoglobin (64 kDa), alpha-fetoprotein (67 kDa) with no symmetry, and streptavidin (52 kDa) at a resolution of 3.5 A, 2.6 A and 2.2 A, respectively. Furthermore, our results demonstrate the potential of UFG for the fields of cryo-electron tomography and structure-based drug discovery.

Uniform thin ice on ultraflat graphene for high-resolution cryo-EM.,Zheng L, Liu N, Gao X, Zhu W, Liu K, Wu C, Yan R, Zhang J, Gao X, Yao Y, Deng B, Xu J, Lu Y, Liu Z, Li M, Wei X, Wang HW, Peng H Nat Methods. 2023 Jan;20(1):123-130. doi: 10.1038/s41592-022-01693-y. Epub 2022 , Dec 15. PMID:36522503<ref>PMID:36522503</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7yim" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Li M]]
[[Category: Liu K]]
[[Category: Liu N]]
[[Category: Liu Z]]
[[Category: Wang HW]]
[[Category: Wang J]]
[[Category: Wu C]]