View source for 7yji


==Crystal structure of Lpg1083 from Legionella pneumophila==
<StructureSection load='7yji' size='340' side='right'caption='[[7yji]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yji]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yji OCA], [https://pdbe.org/7yji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yji RCSB], [https://www.ebi.ac.uk/pdbsum/7yji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yji ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2S6F9N6_LEGPN A0A2S6F9N6_LEGPN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To facilitate its survival, replication, and dissemination, the intracellular pathogen Legionella pneumophila relies on its unique Type IVB secretion system (T4SS) to deliver over 330 effectors to hijack host cell pathways in a spatiotemporal manner. The underlying mechanisms of the effectors and their host targets are largely unexplored due to their little sequence identity to known proteins and functional redundancy. The T4SS effector SidN (Lpg1083) is secreted into host cells during the late infection period. However, to the best of our knowledge, the molecular characterization of SidN has not been previously studied. Herein, we identified SidN as a nuclear envelope-localized effector. Its structure adopts a novel fold and the N-terminal domain is crucial for its specific subcellular localization. Furthermore, we found that SidN is transported by the eukaryotic karyopherin Importin-13 into the nucleus, where it attaches to the N-terminal region of Lamin-B2 to interfere with the integrity of the nuclear envelope, causing nuclear membrane disruption and eventually cell death. Our work provides new insights into the structure and function of an L. pneumophila effector protein, and suggests a potential strategy that the pathogen utilizes to promote cell death and then benefit bacterial escape from the host for secondary infection.

A bacterial effector SidN/Lpg1083 promotes cell death by targeting Lamin-B2.,Gao J, Xu W, Tang F, Xu M, Zhou Q, Yang X, Zhang N, Ma J, Yang Q, Chen X, Qin X, Ge H J Mol Cell Biol. 2023 May 30:mjad036. doi: 10.1093/jmcb/mjad036. PMID:37253620<ref>PMID:37253620</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7yji" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Legionella pneumophila]]
[[Category: Chen X]]
[[Category: Gao J]]
[[Category: Ge H]]