View source for 7ynd


==Cryo-EM structure of Cas7-11-crRNA-Csx29 ternary complex==
<StructureSection load='7ynd' size='340' side='right'caption='[[7ynd]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ynd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YND OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YND FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.29&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ynd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ynd OCA], [https://pdbe.org/7ynd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ynd RCSB], [https://www.ebi.ac.uk/pdbsum/7ynd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ynd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A401FT52_9BACT A0A401FT52_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The recently discovered type III-E CRISPR-Cas effector Cas7-11 shows promise when used as an RNA manipulation tool, but its structure and the mechanisms underlying its function remain unclear. Here we present four cryo-EM structures of Desulfonema ishimotonii Cas7-11-crRNA complex in pre-target and target RNA-bound states, and the cryo-EM structure of DiCas7-11-crRNA bound to its accessory protein DiCsx29. These data reveal structural elements for pre-crRNA processing, target RNA cleavage and regulation. Moreover, a 3' seed region of crRNA is involved in regulating RNA cleavage activity of DiCas7-11-crRNA-Csx29. Our analysis also shows that both the minimal mismatch of 4 nt to the 5' handle of crRNA and the minimal matching of the first 12 nt of the spacer by the target RNA are essential for triggering the protease activity of DiCas7-11-crRNA-Csx29 towards DiCsx30. Taken together, we propose that target RNA recognition and cleavage regulate and fine-tune the protease activity of DiCas7-11-crRNA-Csx29, thus preventing auto-immune responses.

Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector.,Huo Y, Zhao H, Dong Q, Jiang T Nat Microbiol. 2023 Mar;8(3):522-532. doi: 10.1038/s41564-022-01316-4. Epub 2023 , Jan 26. PMID:36702942<ref>PMID:36702942</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ynd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Desulfonema ishimotonii]]
[[Category: Large Structures]]
[[Category: Dong Q]]
[[Category: Huo Y]]
[[Category: Jiang T]]
[[Category: Zhao H]]