View source for 7ypo


==Cryo-EM structure of baculovirus LEF-3 in complex with ssDNA==
<StructureSection load='7ypo' size='340' side='right'caption='[[7ypo]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ypo]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera_nucleopolyhedrovirus Helicoverpa armigera nucleopolyhedrovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypo OCA], [https://pdbe.org/7ypo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypo RCSB], [https://www.ebi.ac.uk/pdbsum/7ypo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q91BW6_9ABAC Q91BW6_9ABAC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Single-stranded DNA-binding proteins (SSBs) interact with single-stranded DNA (ssDNA) to form filamentous structures with various degrees of cooperativity, as a result of intermolecular interactions between neighboring SSB subunits on ssDNA. However, it is still challenging to perform structural studies on SSB-ssDNA filaments at high resolution using the most studied SSB models, largely due to the intrinsic flexibility of these nucleoprotein complexes. In this study, HaLEF-3, an SSB protein from Helicoverpa armigera nucleopolyhedrovirus, was used for in vitro assembly of SSB-ssDNA filaments, which were structurally studied at atomic resolution using cryo-electron microscopy. Combined with the crystal structure of ssDNA-free HaLEF-3 octamers, our results revealed that the three-dimensional rearrangement of HaLEF-3 induced by an internal hinge-bending movement is essential for the formation of helical SSB-ssDNA complexes, while the contacting interface between adjacent HaLEF-3 subunits remains basically intact. We proposed a local cooperative SSB-ssDNA binding model, in which, triggered by exposure to oligonucleotides, HaLEF-3 molecules undergo ring-to-helix transition to initiate continuous SSB-SSB interactions along ssDNA. Unique structural features revealed by the assembly of HaLEF-3 on ssDNA suggest that HaLEF-3 may represent a new class of SSB.

Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA.,Yin J, Fu Y, Rao G, Li Z, Tian K, Chong T, Kuang K, Wang M, Hu Z, Cao S Nucleic Acids Res. 2022 Dec 9;50(22):13100-13113. doi: 10.1093/nar/gkac1142. PMID:36477586<ref>PMID:36477586</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ypo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Helicoverpa armigera nucleopolyhedrovirus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Cao S]]
[[Category: Fu Y]]
[[Category: Li Z]]
[[Category: Rao G]]
[[Category: Yin J]]