View source for 7ywa


==Structure of DinI in complex with RecA filament==
<StructureSection load='7ywa' size='340' side='right'caption='[[7ywa]], [[Resolution|resolution]] 3.26&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ywa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YWA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.26&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ywa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ywa OCA], [https://pdbe.org/7ywa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ywa RCSB], [https://www.ebi.ac.uk/pdbsum/7ywa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ywa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DINI_ECOLI DINI_ECOLI] Involved in SOS regulation. Inhibits RecA by preventing RecA to bind ssDNA. Can displace ssDNA from RecA.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In response to DNA damage, bacterial RecA protein forms filaments with the assistance of DinI protein. The RecA filaments stimulate the autocleavage of LexA, the repressor of more than 50 SOS genes, and activate the SOS response. During the late phase of SOS response, the RecA filaments stimulate the autocleavage of UmuD and lambda repressor CI, leading to mutagenic repair and lytic cycle, respectively. Here, we determined the cryo-electron microscopy structures of Escherichia coli RecA filaments in complex with DinI, LexA, UmuD, and lambdaCI by helical reconstruction. The structures reveal that LexA and UmuD dimers bind in the filament groove and cleave in an intramolecular and an intermolecular manner, respectively, while lambdaCI binds deeply in the filament groove as a monomer. Despite their distinct folds and oligomeric states, all RecA filament binders recognize the same conserved protein features in the filament groove. The SOS response in bacteria can lead to mutagenesis and antimicrobial resistance, and our study paves the way for rational drug design targeting the bacterial SOS response.

Structural basis for regulation of SOS response in bacteria.,Gao B, Liang L, Su L, Wen A, Zhou C, Feng Y Proc Natl Acad Sci U S A. 2023 Jan 10;120(2):e2217493120. doi: , 10.1073/pnas.2217493120. Epub 2023 Jan 4. PMID:36598938<ref>PMID:36598938</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ywa" style="background-color:#fffaf0;"></div>

==See Also==
*[[3D structures of recombinase A|3D structures of recombinase A]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Feng Y]]
[[Category: Gao B]]