View source for 7yzr


==50 mM Rb+ soak of beryllium fluoride inhibited Na+,K+-ATPase, E2-BeFx (rigid body model)==
<StructureSection load='7yzr' size='340' side='right'caption='[[7yzr]], [[Resolution|resolution]] 6.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yzr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YZR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6.92&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzr OCA], [https://pdbe.org/7yzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzr RCSB], [https://www.ebi.ac.uk/pdbsum/7yzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q58K79_PIG Q58K79_PIG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Na(+),K(+)-ATPase generates electrochemical gradients of Na(+) and K(+) across the plasma membrane via a functional cycle that includes various phosphoenzyme intermediates. However, the structure and function of these intermediates and how metal fluorides mimick them require further investigation. Here, we describe a 4.0 A resolution crystal structure and functional properties of the pig kidney Na(+),K(+)-ATPase stabilized by the inhibitor beryllium fluoride (denoted E2-BeF(x)). E2-BeF(x) is expected to mimic properties of the E2P phosphoenzyme, yet with unknown characteristics of ion and ligand binding. The structure resembles the E2P form obtained by phosphorylation from inorganic phosphate (P(i)) and stabilized by cardiotonic steroids, including a low-affinity Mg(2+) site near ion binding site II. Our anomalous Fourier analysis of the crystals soaked in Rb(+) (a K(+) congener) followed by a low-resolution rigid-body refinement (6.9-7.5 A) revealed preocclusion transitions leading to activation of the dephosphorylation reaction. We show that the Mg(2+) location indicates a site of initial K(+) recognition and acceptance upon binding to the outward-open E2P state after Na(+) release. Furthermore, using binding and activity studies, we find that the BeF(x)-inhibited enzyme is also able to bind ADP/ATP and Na(+). These results relate the E2-BeF(x) complex to a transient K(+)- and ADP-sensitive E *P intermediate of the functional cycle of the Na(+),K(+)-ATPase, prior to E2P.

The Na(+),K(+)-ATPase in complex with beryllium fluoride mimics an ATPase phosphorylated state.,Fruergaard MU, Dach I, Andersen JL, Ozol M, Shahsavar A, Quistgaard EM, Poulsen H, Fedosova NU, Nissen P J Biol Chem. 2022 Sep;298(9):102317. doi: 10.1016/j.jbc.2022.102317. Epub 2022 , Aug 2. PMID:35926706<ref>PMID:35926706</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7yzr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Andersen JL]]
[[Category: Dach I]]
[[Category: Fedosova NU]]
[[Category: Fruergaard MU]]
[[Category: Nissen P]]
[[Category: Ozol M]]
[[Category: Poulsen H]]
[[Category: Quistgaard EM]]
[[Category: Shasavar A]]