View source for 7yzy


==pMMO structure from native membranes by cryoET and STA==
<StructureSection load='7yzy' size='340' side='right'caption='[[7yzy]], [[Resolution|resolution]] 4.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yzy]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylococcus_capsulatus_str._Bath Methylococcus capsulatus str. Bath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YZY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yzy OCA], [https://pdbe.org/7yzy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yzy RCSB], [https://www.ebi.ac.uk/pdbsum/7yzy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yzy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PMOA_METCA PMOA_METCA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Methane-oxidizing bacteria play a central role in greenhouse gas mitigation and have potential applications in biomanufacturing. Their primary metabolic enzyme, particulate methane monooxygenase (pMMO), is housed in copper-induced intracytoplasmic membranes (ICMs), of which the function and biogenesis are not known. We show by serial cryo-focused ion beam (cryoFIB) milling/scanning electron microscope (SEM) volume imaging and lamellae-based cellular cryo-electron tomography (cryoET) that these ICMs are derived from the inner cell membrane. The pMMO trimer, resolved by cryoET and subtomogram averaging to 4.8 A in the ICM, forms higher-order hexagonal arrays in intact cells. Array formation correlates with increased enzymatic activity, highlighting the importance of studying the enzyme in its native environment. These findings also demonstrate the power of cryoET to structurally characterize native membrane enzymes in the cellular context.

Structure and activity of particulate methane monooxygenase arrays in methanotrophs.,Zhu Y, Koo CW, Cassidy CK, Spink MC, Ni T, Zanetti-Domingues LC, Bateman B, Martin-Fernandez ML, Shen J, Sheng Y, Song Y, Yang Z, Rosenzweig AC, Zhang P Nat Commun. 2022 Sep 5;13(1):5221. doi: 10.1038/s41467-022-32752-9. PMID:36064719<ref>PMID:36064719</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7yzy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methane monooxygenase 3D structures|Methane monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylococcus capsulatus str. Bath]]
[[Category: Ni T]]
[[Category: Zhang P]]
[[Category: Zhu Y]]