View source for 7z6b


==PET hydrolase PET6 from halophilic organsim Vibrio gazogenes==
<StructureSection load='7z6b' size='340' side='right'caption='[[7z6b]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7z6b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_gazogenes_DSM_21264_=_NBRC_103151 Vibrio gazogenes DSM 21264 = NBRC 103151]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z6B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z6b OCA], [https://pdbe.org/7z6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z6b RCSB], [https://www.ebi.ac.uk/pdbsum/7z6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z6b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1M5F0K3_VIBGA A0A1M5F0K3_VIBGA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The handling of plastic waste and the associated ubiquitous occurrence of microplastic poses one of the biggest challenges of our time. Recent investigations of plastic degrading enzymes have opened new prospects for biological microplastic decomposition as well as recycling applications. For polyethylene terephthalate, in particular, several natural and engineered enzymes are known to have such promising properties. From a previous study that identified new PETase candidates by homology search, we chose the candidate PET6 from the globally distributed, halophilic organism Vibrio gazogenes for further investigation. By mapping the occurrence of Vibrios containing PET6 homologs we demonstrated their ubiquitous prevalence in the pangenome of several Vibrio strains. The biochemical characterization of PET6 showed that PET6 has a comparatively lower activity than other enzymes but also revealed a superior turnover at very high salt concentrations. The crystal structure of PET6 provides structural insights into this adaptation to saline environments. By grafting only a few beneficial mutations from other PET degrading enzymes onto PET6, we increased the activity up to three-fold, demonstrating the evolutionary potential of the enzyme. MD simulations of the variant helped rationalize the mutational effects of those mutants and elucidate the interaction of the enzyme with a PET substrate. With tremendous amounts of plastic waste in the Ocean and the prevalence of Vibrio gazogenes in marine biofilms and estuarine marshes, our findings suggest that Vibrio and the PET6 enzyme are worthy subjects to study the PET degradation in marine environments.

Investigation of the halophilic PET hydrolase PET6 from Vibrio gazogenes.,Weigert S, Perez-Garcia P, Gisdon FJ, Gagsteiger A, Schweinshaut K, Ullmann GM, Chow J, Streit WR, Hocker B Protein Sci. 2022 Dec;31(12):e4500. doi: 10.1002/pro.4500. PMID:36336469<ref>PMID:36336469</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7z6b" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cutinase 3D structures|Cutinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio gazogenes DSM 21264 = NBRC 103151]]
[[Category: Hoecker B]]
[[Category: Weigert S]]