View source for 7zbd


==HaloTag with TRaQ-G ligand==
<StructureSection load='7zbd' size='340' side='right'caption='[[7zbd]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zbd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZBD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ILQ:(10~{R})-7-azanyl-~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-2-cyano-5,5-dimethyl-3-(methylamino)-1-oxidanylidene-spiro[benzo[b][1]benzosiline-10,3-isoindole]-5-carboxamide'>ILQ</scene>, <scene name='pdbligand=ILU:[7-azanyl-10-[5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]-2-(cyanocarbamoyl)phenyl]-5,5-dimethyl-benzo[b][1]benzosilin-3-ylidene]-methyl-azanium'>ILU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zbd OCA], [https://pdbe.org/7zbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zbd RCSB], [https://www.ebi.ac.uk/pdbsum/7zbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zbd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHAA_RHOSO DHAA_RHOSO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glutathione (GSH) is the main determinant of intracellular redox potential and participates in multiple cellular signalling pathways. Achieving a detailed understanding of intracellular GSH homeostasis depends on the development of tools to map GSH compartmentalization and intra-organelle fluctuations. Here we present a GSH-sensing platform for live-cell imaging, termed targetable ratiometric quantitative GSH (TRaQ-G). This chemogenetic sensor possesses a unique reactivity turn-on mechanism, ensuring that the small molecule is only sensitive to GSH in a desired location. Furthermore, TRaQ-G can be fused to a fluorescent protein to give a ratiometric response. Using TRaQ-G fused to a redox-insensitive fluorescent protein, we demonstrate that the nuclear and cytosolic GSH pools are independently regulated during cell proliferation. This sensor was used in combination with a redox-sensitive fluorescent protein to quantify redox potential and GSH concentration simultaneously in the endoplasmic reticulum. Finally, by exchanging the fluorescent protein, we created a near-infrared, targetable and quantitative GSH sensor.

A locally activatable sensor for robust quantification of organellar glutathione.,Emmert S, Quargnali G, Thallmair S, Rivera-Fuentes P Nat Chem. 2023 Oct;15(10):1415-1421. doi: 10.1038/s41557-023-01249-3. Epub 2023 , Jun 15. PMID:37322101<ref>PMID:37322101</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7zbd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodococcus sp]]
[[Category: Emmert S]]
[[Category: Lau K]]
[[Category: Pojer F]]
[[Category: Rivera-Fuentes P]]