View source for 7zbt


==Subtomogram averaging of Rubisco from native Halothiobacillus carboxysomes==
<StructureSection load='7zbt' size='340' side='right'caption='[[7zbt]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zbt]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothiobacillus_neapolitanus Halothiobacillus neapolitanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZBT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zbt OCA], [https://pdbe.org/7zbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zbt RCSB], [https://www.ebi.ac.uk/pdbsum/7zbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zbt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RBL1_HALNC RBL1_HALNC] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
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== Publication Abstract from PubMed ==
Carboxysomes are a family of bacterial microcompartments in cyanobacteria and chemoautotrophs. They encapsulate Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase catalyzing carbon fixation inside a proteinaceous shell. How Rubisco complexes pack within the carboxysomes is unknown. Using cryo-electron tomography, we determine the distinct 3D organization of Rubisco inside two distant alpha-carboxysomes from a marine alpha-cyanobacterium Cyanobium sp. PCC 7001 where Rubiscos are organized in three concentric layers, and from a chemoautotrophic bacterium Halothiobacillus neapolitanus where they form intertwining spirals. We further resolve the structures of native Rubisco as well as its higher-order assembly at near-atomic resolutions by subtomogram averaging. The structures surprisingly reveal that the authentic intrinsically disordered linker protein CsoS2 interacts with Rubiscos in native carboxysomes but functions distinctively in the two alpha-carboxysomes. In contrast to the uniform Rubisco-CsoS2 association in the Cyanobium alpha-carboxysome, CsoS2 binds only to the Rubiscos close to the shell in the Halo alpha-carboxysome. Our findings provide critical knowledge of the assembly principles of alpha-carboxysomes, which may aid in the rational design and repurposing of carboxysome structures for new functions.

Structure and assembly of cargo Rubisco in two native alpha-carboxysomes.,Ni T, Sun Y, Burn W, Al-Hazeem MMJ, Zhu Y, Yu X, Liu LN, Zhang P Nat Commun. 2022 Jul 25;13(1):4299. doi: 10.1038/s41467-022-32004-w. PMID:35879301<ref>PMID:35879301</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7zbt" style="background-color:#fffaf0;"></div>

==See Also==
*[[RuBisCO 3D structures|RuBisCO 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halothiobacillus neapolitanus]]
[[Category: Large Structures]]
[[Category: Liu L]]
[[Category: Ni T]]
[[Category: Sun Y]]
[[Category: Yu X]]
[[Category: Zhang P]]
[[Category: Zhu Y]]