View source for 7zlv


==Tail tip of siphophage T5 : central fibre protein pb4==
<StructureSection load='7zlv' size='340' side='right'caption='[[7zlv]], [[Resolution|resolution]] 4.22&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zlv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T5 Escherichia phage T5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZLV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.22&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zlv OCA], [https://pdbe.org/7zlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zlv RCSB], [https://www.ebi.ac.uk/pdbsum/7zlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zlv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FIBC_BPT5 FIBC_BPT5] Together with BHP-pb3, forms the central straight fiber, bearing the receptor binding protein RBP-pb5 at its C-terminus (PubMed:36961893). Upon RBP-pb5 binding to the host receptor FhuA, the central fiber reorganizes and undergoes structural rearrangements that lead to its bending, bringing BHP-pb3 close to the host membrane for anchoring (PubMed:36961893).<ref>PMID:36961893</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a long flexible tail (Siphoviridae) at the tip of which receptor binding proteins (RBPs) specifically interact with their host, triggering infection. In siphophage T5, the unique RBP is located at the extremity of a central fiber. We present the structures of T5 tail tip, determined by cryo-electron microscopy before and after interaction with its E. coli receptor, FhuA, reconstituted into nanodisc. These structures bring out the important conformational changes undergone by T5 tail tip upon infection, which include bending of T5 central fiber on the side of the tail tip, tail anchoring to the membrane, tail tube opening, and formation of a transmembrane channel. The data allow to detail the first steps of an otherwise undescribed infection mechanism.

Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation.,Linares R, Arnaud CA, Effantin G, Darnault C, Epalle NH, Boeri Erba E, Schoehn G, Breyton C Sci Adv. 2023 Mar 24;9(12):eade9674. doi: 10.1126/sciadv.ade9674. Epub 2023 Mar , 24. PMID:36961893<ref>PMID:36961893</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7zlv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia phage T5]]
[[Category: Large Structures]]
[[Category: Arnaud CA]]
[[Category: Boeri Erba E]]
[[Category: Breyton C]]
[[Category: Effantin G]]
[[Category: Epalle N]]
[[Category: Linares R]]
[[Category: Schoehn G]]