View source for 7zrh


==Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+==
<StructureSection load='7zrh' size='340' side='right'caption='[[7zrh]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zrh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZRH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zrh OCA], [https://pdbe.org/7zrh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zrh RCSB], [https://www.ebi.ac.uk/pdbsum/7zrh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zrh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KDPA_ECOLI KDPA_ECOLI] Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm (PubMed:2849541, PubMed:8499455, PubMed:23930894). This subunit binds and transports the potassium across the cytoplasmic membrane (PubMed:7896809).<ref>PMID:23930894</ref> <ref>PMID:2849541</ref> <ref>PMID:7896809</ref> <ref>PMID:8499455</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
KdpFABC is a high-affinity prokaryotic K(+) uptake system that forms a functional chimera between a channel-like subunit (KdpA) and a P-type ATPase (KdpB). At high K(+) levels, KdpFABC needs to be inhibited to prevent excessive K(+) accumulation to the point of toxicity. This is achieved by a phosphorylation of the serine residue in the TGES(162) motif in the A domain of the pump subunit KdpB (KdpB(S162-P)). Here, we explore the structural basis of inhibition by KdpB(S162) phosphorylation by determining the conformational landscape of KdpFABC under inhibiting and non-inhibiting conditions. Under turnover conditions, we identified a new inhibited KdpFABC state that we termed E1P tight, which is not part of the canonical Post-Albers transport cycle of P-type ATPases. It likely represents the biochemically described stalled E1P state adopted by KdpFABC upon KdpB(S162) phosphorylation. The E1P tight state exhibits a compact fold of the three cytoplasmic domains and is likely adopted when the transition from high-energy E1P states to E2P states is unsuccessful. This study represents a structural characterization of a biologically relevant off-cycle state in the P-type ATPase family and supports the emerging discussion of P-type ATPase regulation by such states.

Inhibited KdpFABC transitions into an E1 off-cycle state.,Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C Elife. 2022 Oct 18;11:e80988. doi: 10.7554/eLife.80988. PMID:36255052<ref>PMID:36255052</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7zrh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Corey RA]]
[[Category: Haenelt I]]
[[Category: Hielkema L]]
[[Category: Paulino C]]
[[Category: Silberberg JM]]
[[Category: Stansfeld PJ]]
[[Category: Stock C]]
[[Category: Wunnicke D]]