View source for 7zxv


==Orange Carotenoid Protein Trp-288 BTA mutant==
<StructureSection load='7zxv' size='340' side='right'caption='[[7zxv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zxv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZXV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=45D:BETA,BETA-CAROTENE-4,4-DIONE'>45D</scene>, <scene name='pdbligand=4OG:3-(1-BENZOTHIOPHEN-3-YL)-L-ALANINE'>4OG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zxv OCA], [https://pdbe.org/7zxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zxv RCSB], [https://www.ebi.ac.uk/pdbsum/7zxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zxv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OCP_SYNY3 OCP_SYNY3] Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.<ref>PMID:16531492</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Introduction: Dissecting the intricate networks of covalent and non-covalent interactions that stabilize complex protein structures is notoriously difficult and requires subtle atomic-level exchanges to precisely affect local chemical functionality. The function of the Orange Carotenoid Protein (OCP), a light-driven photoswitch involved in cyanobacterial photoprotection, depends strongly on two H-bonds between the 4-ketolated xanthophyll cofactor and two highly conserved residues in the C-terminal domain (Trp288 and Tyr201). Method: By orthogonal translation, we replaced Trp288 in Synechocystis OCP with 3-benzothienyl-L-alanine (BTA), thereby exchanging the imino nitrogen for a sulphur atom. Results: Although the high-resolution (1.8 A) crystal structure of the fully photoactive OCP-W288_BTA protein showed perfect isomorphism to the native structure, the spectroscopic and kinetic properties changed distinctly. We accurately parameterized the effects of the absence of a single H-bond on the spectroscopic and thermodynamic properties of OCP photoconversion and reveal general principles underlying the design of photoreceptors by natural evolution. Discussion: Such "molecular surgery" is superior over trial-and-error methods in hypothesis-driven research of complex chemical systems.

Parameterization of a single H-bond in Orange Carotenoid Protein by atomic mutation reveals principles of evolutionary design of complex chemical photosystems.,Moldenhauer M, Tseng HW, Kraskov A, Tavraz NN, Yaroshevich IA, Hildebrandt P, Sluchanko NN, Hochberg GA, Essen LO, Budisa N, Korf L, Maksimov EG, Friedrich T Front Mol Biosci. 2023 Jan 26;10:1072606. doi: 10.3389/fmolb.2023.1072606. , eCollection 2023. PMID:36776742<ref>PMID:36776742</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7zxv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Budisa N]]
[[Category: Essen L-O]]
[[Category: Friedrich T]]
[[Category: Hildebrandt P]]
[[Category: Hochberg G]]
[[Category: Korf L]]
[[Category: Kraskov A]]
[[Category: Maksimov EG]]
[[Category: Moldenhauer M]]
[[Category: Tavraz NN]]
[[Category: Tseng H-W]]