View source for 8a60


==Crystal structure of FhuA in complex with the superinfection exclusion lipoprotein Llp==
<StructureSection load='8a60' size='340' side='right'caption='[[8a60]], [[Resolution|resolution]] 3.37&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8a60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_phage_T5 Escherichia phage T5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A60 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.37&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a60 OCA], [https://pdbe.org/8a60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a60 RCSB], [https://www.ebi.ac.uk/pdbsum/8a60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a60 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LLP_BPT5 LLP_BPT5] Inhibits of the adsorption of T5 to its FhuA receptor. By this means, T5 within cells excludes superinfection by T5 and other phages using FhuA receptor as a ligand.<ref>PMID:8045901</ref> <ref>PMID:8057856</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A key but poorly understood stage of the bacteriophage life cycle is the binding of phage receptor-binding proteins (RBPs) to receptors on the host cell surface, leading to injection of the phage genome and, for lytic phages, host cell lysis. To prevent secondary infection by the same or a closely related phage and nonproductive phage adsorption to lysed cell fragments, superinfection exclusion (SE) proteins can prevent the binding of RBPs via modulation of the host receptor structure in ways that are also unclear. Here, we present the cryogenic electron microscopy (cryo-EM) structure of the phage T5 outer membrane (OM) receptor FhuA in complex with the T5 RBP pb5, and the crystal structure of FhuA complexed to the OM SE lipoprotein Llp. Pb5 inserts four loops deeply into the extracellular lumen of FhuA and contacts the plug but does not cause any conformational changes in the receptor, supporting the view that DNA translocation does not occur through the lumen of OM channels. The FhuA-Llp structure reveals that Llp is periplasmic and binds to a nonnative conformation of the plug of FhuA, causing the inward folding of two extracellular loops via "reverse" allostery. The inward-folded loops of FhuA overlap with the pb5 binding site, explaining how Llp binding to FhuA abolishes further infection of Escherichia coli by phage T5 and suggesting a mechanism for SE via the jamming of TonB-dependent transporters by small phage lipoproteins.

Structural basis for host recognition and superinfection exclusion by bacteriophage T5.,van den Berg B, Silale A, Basle A, Brandner AF, Mader SL, Khalid S Proc Natl Acad Sci U S A. 2022 Oct 18;119(42):e2211672119. doi: , 10.1073/pnas.2211672119. Epub 2022 Oct 10. PMID:36215462<ref>PMID:36215462</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8a60" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Escherichia phage T5]]
[[Category: Large Structures]]
[[Category: Van den Berg B]]