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==Single Particle cryo-EM of the lipid binding protein P116 (MPN213) from Mycoplasma pneumoniae at 3.3 Angstrom resolution.==
<StructureSection load='8a9a' size='340' side='right'caption='[[8a9a]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8a9a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasma_pneumoniae_M129 Mycoplasma pneumoniae M129]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A9A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a9a OCA], [https://pdbe.org/8a9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a9a RCSB], [https://www.ebi.ac.uk/pdbsum/8a9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a9a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y213_MYCPN Y213_MYCPN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213). Single-particle cryo-electron microscopy of P116 reveals a homodimer presenting a previously unseen fold, forming a huge hydrophobic cavity, which is fully accessible to solvent. Lipidomics analysis shows that P116 specifically extracts lipids such as phosphatidylcholine, sphingomyelin and cholesterol. Structures of different conformational states reveal the mechanism by which lipids are extracted. This finding immediately suggests a way to control Mycoplasma infection by interfering with lipid uptake.

Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas.,Sprankel L, Vizarraga D, Martin J, Manger S, Meier-Credo J, Marcos M, Julve J, Rotllan N, Scheffer MP, Escola-Gil JC, Langer JD, Pinol J, Fita I, Frangakis AS Nat Struct Mol Biol. 2023 Mar;30(3):321-329. doi: 10.1038/s41594-023-00922-y. , Epub 2023 Feb 13. PMID:36782049<ref>PMID:36782049</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8a9a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycoplasma pneumoniae M129]]
[[Category: Sprankel L]]
[[Category: Vizarraga D]]