View source for 8ag3


==Vaccinia C16 N-terminal domains==
<StructureSection load='8ag3' size='340' side='right'caption='[[8ag3]], [[Resolution|resolution]] 3.47&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ag3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_Western_Reserve Vaccinia virus Western Reserve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AG3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.47&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ag3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ag3 OCA], [https://pdbe.org/8ag3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ag3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ag3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ag3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C10_VACCW C10_VACCW] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Detection of cytosolic DNA is a central element of the innate immunity system against viral infection. The Ku heterodimer, a component of the NHEJ pathway of DNA repair in the nucleus, functions as DNA sensor that detects dsDNA of viruses that replicate in the cytoplasm. Vaccinia virus expresses two proteins, C4 and C16, that inactivate DNA sensing and enhance virulence. The structural basis for this is unknown. Here we determine the structure of the C16 - Ku complex using cryoEM. Ku binds dsDNA by a preformed ring but C16 sterically blocks this access route, abrogating binding to a dsDNA end and its insertion into DNA-PK, thereby averting signalling into the downstream innate immunity system. C4 replicates these activities using a domain with 54% identity to C16. Our results reveal how vaccinia virus subverts the capacity of Ku to recognize viral DNA.

Structural basis for the inactivation of cytosolic DNA sensing by the vaccinia virus.,Rivera-Calzada A, Arribas-Bosacoma R, Ruiz-Ramos A, Escudero-Bravo P, Boskovic J, Fernandez-Leiro R, Oliver AW, Pearl LH, Llorca O Nat Commun. 2022 Nov 18;13(1):7062. doi: 10.1038/s41467-022-34843-z. PMID:36400800<ref>PMID:36400800</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ag3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Vaccinia virus Western Reserve]]
[[Category: Arribas-Bosacoma R]]
[[Category: Llorca O]]
[[Category: Pearl LH]]
[[Category: Rivera-Calzada A]]