View source for 8ahz


==Native VirD of Streptomyces virginiae==
<StructureSection load='8ahz' size='340' side='right'caption='[[8ahz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ahz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_virginiae Streptomyces virginiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AHZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ahz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ahz OCA], [https://pdbe.org/8ahz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ahz RCSB], [https://www.ebi.ac.uk/pdbsum/8ahz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ahz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4PHM7_STRVG A4PHM7_STRVG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During biosynthesis by multi-modular trans-AT polyketide synthases, polyketide structural space can be expanded by conversion of initially-formed electrophilic beta-ketones into beta-alkyl groups. These multi-step transformations are catalysed by 3-hydroxy-3-methylgluratryl synthase cassettes of enzymes. While mechanistic aspects of these reactions have been delineated, little information is available concerning how the cassettes select the specific polyketide intermediate(s) to target. Here we use integrative structural biology to identify the basis for substrate choice in module 5 of the virginiamycin M trans-AT polyketide synthase. Additionally, we show in vitro that module 7, at minimum, is a potential additional site for beta-methylation. Indeed, analysis by HPLC-MS coupled with isotopic labelling and pathway inactivation identifies a metabolite bearing a second beta-methyl at the expected position. Collectively, our results demonstrate that several control mechanisms acting in concert underpin beta-branching programming. Furthermore, variations in this control - whether natural or by design - open up avenues for diversifying polyketide structures towards high-value derivatives.

Decrypting the programming of beta-methylation in virginiamycin M biosynthesis.,Collin S, Cox RJ, Paris C, Jacob C, Chagot B, Weissman KJ, Gruez A Nat Commun. 2023 Mar 10;14(1):1327. doi: 10.1038/s41467-023-36974-3. PMID:36899003<ref>PMID:36899003</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ahz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces virginiae]]
[[Category: Collin S]]
[[Category: Gruez A]]