View source for 8apy


==Crystal structure of the H12A variant of the KDEL receptor bound to sybody==
<StructureSection load='8apy' size='340' side='right'caption='[[8apy]], [[Resolution|resolution]] 2.34&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8apy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8APY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8APY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.34&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8apy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8apy OCA], [https://pdbe.org/8apy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8apy RCSB], [https://www.ebi.ac.uk/pdbsum/8apy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8apy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ERD22_CHICK ERD22_CHICK] Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Trafficking receptors control protein localization through the recognition of specific signal sequences that specify unique cellular locations. Differences in luminal pH are important for the vectorial trafficking of cargo receptors. The KDEL receptor is responsible for maintaining the integrity of the ER by retrieving luminally localized folding chaperones in a pH-dependent mechanism. Structural studies have revealed the end states of KDEL receptor activation and the mechanism of selective cargo binding. However, precisely how the KDEL receptor responds to changes in luminal pH remains unclear. To explain the mechanism of pH sensing, we combine analysis of X-ray crystal structures of the KDEL receptor at neutral and acidic pH with advanced computational methods and cell-based assays. We show a critical role for ordered water molecules that allows us to infer a direct connection between protonation in different cellular compartments and the consequent changes in the affinity of the receptor for cargo.

Molecular basis for pH sensing in the KDEL trafficking receptor.,Wu Z, Smith K, Gerondopoulos A, Sobajima T, Parker JL, Barr FA, Newstead S, Biggin PC Structure. 2024 Jul 11;32(7):866-877.e4. doi: 10.1016/j.str.2024.03.013. Epub , 2024 Apr 15. PMID:38626766<ref>PMID:38626766</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8apy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Newstead S]]
[[Category: Parker JL]]
[[Category: Smith K]]