View source for 8as8


==E. coli Wadjet JetABC monomer==
<StructureSection load='8as8' size='340' side='right'caption='[[8as8]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8as8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AS8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8as8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8as8 OCA], [https://pdbe.org/8as8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8as8 RCSB], [https://www.ebi.ac.uk/pdbsum/8as8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8as8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A5F1WGW3_ECOLX A0A5F1WGW3_ECOLX] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Structural maintenance of chromosome (SMC) complexes fold DNA by loop extrusion to support chromosome segregation and genome maintenance. Wadjet systems (JetABCD/MksBEFG/EptABCD) are derivative SMC complexes with roles in bacterial immunity against selfish DNA. Here, we show that JetABCD restricts circular plasmids with an upper size limit of about 100 kb, whereas a linear plasmid evades restriction. Purified JetABCD complexes cleave circular DNA molecules, regardless of the DNA helical topology; cleavage is DNA sequence nonspecific and depends on the SMC ATPase. A cryo-EM structure reveals a distinct JetABC dimer-of-dimers geometry, with the two SMC dimers facing in opposite direction-rather than the same as observed with MukBEF. We hypothesize that JetABCD is a DNA-shape-specific endonuclease and propose the "total extrusion model" for DNA cleavage exclusively when extrusion of an entire plasmid has been completed by a JetABCD complex. Total extrusion cannot be achieved on the larger chromosome, explaining how self-DNA may evade processing.

DNA-measuring Wadjet SMC ATPases restrict smaller circular plasmids by DNA cleavage.,Liu HW, Roisne-Hamelin F, Beckert B, Li Y, Myasnikov A, Gruber S Mol Cell. 2022 Dec 15;82(24):4727-4740.e6. doi: 10.1016/j.molcel.2022.11.015. PMID:36525956<ref>PMID:36525956</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8as8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Beckert B]]
[[Category: Gruber S]]
[[Category: Li Y]]
[[Category: Myasnikov A]]
[[Category: Roisne-Hamelin F]]