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==Staphylococcus aureus endonuclease IV orthorhombic crystal form==
<StructureSection load='8axy' size='340' side='right'caption='[[8axy]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8axy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AXY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8axy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8axy OCA], [https://pdbe.org/8axy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8axy RCSB], [https://www.ebi.ac.uk/pdbsum/8axy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8axy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/END4_STAAR END4_STAAR] Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During Staphylococcus aureus infections, reactive oxygen species cause DNA damage, including nucleotide base modification. After removal of the defective base, excision repair requires an endonuclease IV (Nfo), which hydrolyzes the phosphodiester bond 5' to the abasic nucleotide. This class of enzymes, typified by the enzyme from Escherichia coli, contains a catalytic site with three metal ions, previously reported to be all Zn(2+). The 1.05 A structure of Nfo from the Gram-positive organism S. aureus (SaNfo) revealed two inner Fe(2+) ions and one Zn(2+) as confirmed by dispersive anomalous difference maps. SaNfo has a previously undescribed water molecule liganded to Fe(1) forming an octahedral coordination geometry and hydrogen bonded to Tyr33, an active site residue conserved in many Gram-positive bacteria, but which is Phe in Gram-negative species that coordinate Zn(2+) at the corresponding site. The 1.9 A structure of E. coli Nfo (EcNfo), purified without added metals, revealed that metal 2 is Fe(2+) and not Zn(2+). Octahedral coordination for the sites occupied by Fe(2+) suggests a stereoselective mechanism for differentiating between Fe(2+) and Zn(2+) in this enzyme class. Kinetics and an inhibitor competition assay of SaNfo reveal product inhibition (or slow product release), especially at low ionic strength, caused in part by a Lys-rich DNA binding loop present in SaNfo and Gram-positive species but not in EcNfo. Biological significance of the slow product release is discussed. Catalytic activity in vitro is optimal at 300 mM NaCl, which is consistent with the halotolerant phenotype of S. aureus.

Octahedral Iron in Catalytic Sites of Endonuclease IV from Staphylococcus aureus and Escherichia coli.,Kirillov S, Isupov M, Paterson NG, Wiener R, Abeldenov S, Saper MA, Rouvinski A Biochemistry. 2024 Dec 10. doi: 10.1021/acs.biochem.4c00447. PMID:39655415<ref>PMID:39655415</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8axy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Isupov MN]]
[[Category: Kirillov S]]
[[Category: Rouvinski A]]
[[Category: Saper MA]]
[[Category: Wiener R]]