View source for 8bdw


==Crystal structure of CnaB2 domain from Lactobacillus plantarum==
<StructureSection load='8bdw' size='340' side='right'caption='[[8bdw]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8bdw]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BDW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bdw OCA], [https://pdbe.org/8bdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bdw RCSB], [https://www.ebi.ac.uk/pdbsum/8bdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bdw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F9UR90_LACPL F9UR90_LACPL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A type of protein/peptide pair known as Catcher/Tag pair spontaneously forms an intermolecular isopeptide bond which can be applied for biomolecular click reactions. Covalent protein conjugation using Catcher/Tag pairs has turned out to be a valuable tool in biotechnology and biomedicines, but it is essential to increase the current toolbox of orthogonal Catcher/Tag pairs to expand the range of applications further, for example, for controlled multiple-fragment ligation. We report here the engineering of novel Catcher/Tag pairs for protein ligation, aided by a crystal structure of a minimal CnaB domain from Lactobacillus plantarum. We show that a newly engineered pair, called SilkCatcher/Tag enables efficient pH-inducible protein ligation in addition to being compatible with the widely used SpyCatcher/Tag pair. Finally, we demonstrate the use of the SilkCatcher/Tag pair in the production of native-sized highly repetitive spider-silk-like proteins with &gt;90 % purity, which is not possible by traditional recombinant production methods.

Biomolecular Click Reactions Using a Minimal pH-Activated Catcher/Tag Pair for Producing Native-Sized Spider-Silk Proteins.,Fan R, Hakanpaa J, Elfving K, Taberman H, Linder MB, Aranko AS Angew Chem Int Ed Engl. 2023 Mar 6;62(11):e202216371. doi: , 10.1002/anie.202216371. Epub 2023 Feb 7. PMID:36695475<ref>PMID:36695475</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8bdw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lactiplantibacillus plantarum]]
[[Category: Large Structures]]
[[Category: Aranko AS]]
[[Category: Hakanpaa J]]
[[Category: Linder MB]]
[[Category: Taberman H]]