View source for 8bh1


==Core divisome complex FtsWIQBL from Pseudomonas aeruginosa==
<StructureSection load='8bh1' size='340' side='right'caption='[[8bh1]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8bh1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BH1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bh1 OCA], [https://pdbe.org/8bh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bh1 RCSB], [https://www.ebi.ac.uk/pdbsum/8bh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bh1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HW00_PSEAE Q9HW00_PSEAE] Peptidoglycan polymerase that is essential for cell division.[HAMAP-Rule:MF_00913]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In most bacteria, cell division relies on the synthesis of new cell wall material by the multiprotein divisome complex. Thus, at the core of the divisome are the transglycosylase FtsW, which synthesises peptidoglycan strands from its substrate Lipid II, and the transpeptidase FtsI that cross-links these strands to form a mesh, shaping and protecting the bacterial cell. The FtsQ-FtsB-FtsL trimeric complex interacts with the FtsWI complex and is involved in regulating its enzymatic activities; however, the structure of this pentameric complex is unknown. Here, we present the cryogenic electron microscopy structure of the FtsWIQBL complex from Pseudomonas aeruginosa at 3.7 A resolution. Our work reveals intricate structural details, including an extended coiled coil formed by FtsL and FtsB and the periplasmic interaction site between FtsL and FtsI. Our structure explains the consequences of previously reported mutations and we postulate a possible activation mechanism involving a large conformational change in the periplasmic domain. As FtsWIQBL is central to the divisome, our structure is foundational for the design of future experiments elucidating the precise mechanism of bacterial cell division, an important antibiotic target.

Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL.,Kashammer L, van den Ent F, Jeffery M, Jean NL, Hale VL, Lowe J Nat Microbiol. 2023 Jun;8(6):1149-1159. doi: 10.1038/s41564-023-01368-0. Epub , 2023 May 1. PMID:37127704<ref>PMID:37127704</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8bh1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Jeffery M]]
[[Category: Kaeshammer L]]
[[Category: Lowe J]]
[[Category: Van den Ent F]]