View source for 8bii


==O-Methyltransferase Plu4895 (mutant H229N) in complex with SAH==
<StructureSection load='8bii' size='340' side='right'caption='[[8bii]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8bii]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_laumondii_subsp._laumondii_TTO1 Photorhabdus laumondii subsp. laumondii TTO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BII FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bii OCA], [https://pdbe.org/8bii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bii RCSB], [https://www.ebi.ac.uk/pdbsum/8bii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bii ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7MY04_PHOLL Q7MY04_PHOLL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Modification of the polyketide anthraquinone AQ-256 in the entomopathogenic Photorhabdus luminescens involves several O-methylations, but the biosynthetic gene cluster antA-I lacks corresponding tailoring enzymes. We here describe the identification of five putative, highly homologous O-methyltransferases encoded in the genome of P. luminescens. Activity assays in vitro and deletion experiments in vivo revealed that three of them account for anthraquinone tailoring by producing three monomethylated and two dimethylated species of AQ-256. X-ray structures of all five enzymes indicate high structural and mechanistic similarity. As confirmed by structure-based mutagenesis, a conserved histidine at the active site likely functions as a general base for substrate deprotonation and subsequent methyl transfer in all enzymes. Eight complex structures with AQ-256 as well as mono- and dimethylated derivatives confirm the substrate specificity patterns found in vitro and visualize how single amino acid differences in the active-site pockets impact substrate orientation and govern site-specific methylation.

A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments.,Huber EM, Kreling L, Heinrich AK, Dunnebacke M, Pothig A, Bode HB, Groll M Structure. 2023 May 4;31(5):573-583.e5. doi: 10.1016/j.str.2023.03.001. Epub 2023 , Mar 23. PMID:36963398<ref>PMID:36963398</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8bii" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photorhabdus laumondii subsp. laumondii TTO1]]
[[Category: Groll M]]
[[Category: Huber EM]]