View source for 8bmw


==SsoCsm==
<StructureSection load='8bmw' size='340' side='right'caption='[[8bmw]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8bmw]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BMW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bmw OCA], [https://pdbe.org/8bmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bmw RCSB], [https://www.ebi.ac.uk/pdbsum/8bmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bmw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q97YA8_SACS2 Q97YA8_SACS2] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a range of enzymatic activities and downstream ancillary effectors. The broad array of current biotechnological CRISPR applications is mainly based on proteins classified as Type II, however recent developments established the feasibility and efficacy of multi-protein Type III CRISPR-Cas effector complexes as RNA-targeting tools in eukaryotes. The crenarchaeon Saccharolobus solfataricus has two type III system subtypes (III-B and III-D). Here, we report the cryo-EM structure of the Csm Type III-D complex from S. solfataricus (SsoCsm), which uses CRISPR RNA to bind target RNA molecules, activating the Cas10 subunit for antiviral defence. The structure reveals the complex organisation, subunit/subunit connectivity and protein/guide RNA interactions of the SsoCsm complex, one of the largest CRISPR effectors known.

Structure of the Saccharolobus solfataricus type III-D CRISPR effector.,Cannone G, Kompaniiets D, Graham S, White MF, Spagnolo L Curr Res Struct Biol. 2023 Feb 10;5:100098. doi: 10.1016/j.crstbi.2023.100098. , eCollection 2023. PMID:36843655<ref>PMID:36843655</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8bmw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharolobus solfataricus]]
[[Category: Spagnolo L]]
[[Category: White MF]]