View source for 8cad


==Cryo-EM structure of the Ceres homohexamer from Galleria mellonella saliva==
<StructureSection load='8cad' size='340' side='right'caption='[[8cad]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8cad]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Galleria_mellonella Galleria mellonella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CAD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cad OCA], [https://pdbe.org/8cad PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cad RCSB], [https://www.ebi.ac.uk/pdbsum/8cad PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cad ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A6J1WN20_GALME A0A6J1WN20_GALME] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran Galleria mellonella larvae is capable of oxidizing and depolymerizing polyethylene in hours at room temperature. Here, we analyze by cryo-electron microscopy (cryo-EM) G. mellonella's saliva directly from the native source. The three-dimensional reconstructions reveal that the buccal secretion is mainly composed of four hexamerins belonging to the hemocyanin/phenoloxidase family, renamed Demetra, Cibeles, Ceres, and a previously unidentified factor termed Cora. Functional assays show that this factor, as its counterparts Demetra and Ceres, is also able to oxidize and degrade polyethylene. The cryo-EM data and the x-ray analysis from purified fractions show that they self-assemble primarily into three macromolecular complexes with striking structural differences that likely modulate their activity. Overall, these results establish the ground to further explore the hexamerins' functionalities, their role in vivo, and their eventual biotechnological application.

Plastic degradation by insect hexamerins: Near-atomic resolution structures of the polyethylene-degrading proteins from the wax worm saliva.,Spinola-Amilibia M, Illanes-Vicioso R, Ruiz-Lopez E, Colomer-Vidal P, Rodriguez-Ventura F, Peces Perez R, Arias CF, Torroba T, Sola M, Arias-Palomo E, Bertocchini F Sci Adv. 2023 Sep 22;9(38):eadi6813. doi: 10.1126/sciadv.adi6813. Epub 2023 Sep , 20. PMID:37729416<ref>PMID:37729416</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8cad" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Galleria mellonella]]
[[Category: Large Structures]]
[[Category: Arias-Palomo E]]
[[Category: Spinola-Amilibia M]]