View source for 8cd1


==70S-PHIKZ014==
<StructureSection load='8cd1' size='340' side='right'caption='[[8cd1]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8cd1]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CD1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CD1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cd1 OCA], [https://pdbe.org/8cd1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cd1 RCSB], [https://www.ebi.ac.uk/pdbsum/8cd1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cd1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL32_PSEAE RL32_PSEAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteriophages must seize control of the host gene expression machinery to replicate. To bypass bacterial anti-phage defence systems, this host takeover occurs immediately upon infection. A general understanding of phage mechanisms for immediate targeting of host transcription and translation processes is lacking. Here we introduce an integrative high-throughput approach to uncover phage-encoded proteins that target the gene expression machinery of Pseudomonas aeruginosa immediately upon infection with the jumbo phage PhiKZ. By integrating biochemical, genetic and structural analyses, we identify an abundant and conserved phage factor PhiKZ014 that targets the large ribosomal subunit by binding the 5S ribosomal RNA, and rapidly promotes replication in several clinical isolates. PhiKZ014 is among the earliest PhiKZ proteins expressed after infection and remains bound to ribosomes during the entire translation cycle. Our study provides a strategy to decipher molecular components of phage-mediated host takeover and argues that phage genomes represent an untapped discovery space for proteins that modulate the host gene expression machinery.

Phage proteins target and co-opt host ribosomes immediately upon infection.,Gerovac M, Chihara K, Wicke L, Bottcher B, Lavigne R, Vogel J Nat Microbiol. 2024 Mar;9(3):787-800. doi: 10.1038/s41564-024-01616-x. Epub 2024 , Mar 4. PMID:38443577<ref>PMID:38443577</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8cd1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Gerovac M]]
[[Category: Vogel J]]