View source for 8cee


==Rnase R bound to a 30S degradation intermediate (State I - head-turning)==
<StructureSection load='8cee' size='340' side='right'caption='[[8cee]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8cee]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CEE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cee OCA], [https://pdbe.org/8cee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cee RCSB], [https://www.ebi.ac.uk/pdbsum/8cee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cee ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNR_BACSU RNR_BACSU] 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (By similarity). Involved in degradation of type I toxin-antitoxin system bsrG/SR4 RNAs (PubMed:22229825).[HAMAP-Rule:MF_01895]<ref>PMID:22229825</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein synthesis is a major energy-consuming process of the cell that requires the controlled production(1-3) and turnover(4,5) of ribosomes. Although the past few years have seen major advances in our understanding of ribosome biogenesis, structural insight into the degradation of ribosomes has been lacking. Here we present native structures of two distinct small ribosomal 30S subunit degradation intermediates associated with the 3' to 5' exonuclease ribonuclease R (RNase R). The structures reveal that RNase R binds at first to the 30S platform to facilitate the degradation of the functionally important anti-Shine-Dalgarno sequence and the decoding-site helix 44. RNase R then encounters a roadblock when it reaches the neck region of the 30S subunit, and this is overcome by a major structural rearrangement of the 30S head, involving the loss of ribosomal proteins. RNase R parallels this movement and relocates to the decoding site by using its N-terminal helix-turn-helix domain as an anchor. In vitro degradation assays suggest that head rearrangement poses a major kinetic barrier for RNase R, but also indicate that the enzyme alone is sufficient for complete degradation of 30S subunits. Collectively, our results provide a mechanistic basis for the degradation of 30S mediated by RNase R, and reveal that RNase R targets orphaned 30S subunits using a dynamic mechanism involving an anchored switching of binding sites.

Structural basis of ribosomal 30S subunit degradation by RNase R.,Dimitrova-Paternoga L, Kasvandik S, Beckert B, Granneman S, Tenson T, Wilson DN, Paternoga H Nature. 2024 Feb;626(8001):1133-1140. doi: 10.1038/s41586-024-07027-6. Epub 2024 , Feb 7. PMID:38326618<ref>PMID:38326618</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8cee" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Dimitrova-Paternoga L]]
[[Category: Paternoga H]]
[[Category: Wilson DN]]