View source for 8cli


==TFIIIC TauB-DNA monomer==
<StructureSection load='8cli' size='340' side='right'caption='[[8cli]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8cli]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CLI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cli OCA], [https://pdbe.org/8cli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cli RCSB], [https://www.ebi.ac.uk/pdbsum/8cli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cli ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TF3C1_HUMAN TF3C1_HUMAN] Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. Binds to the box B promoter element.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules tauA and tauB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The tauB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both tauA and tauB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation.

Structural insights into human TFIIIC promoter recognition.,Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW Sci Adv. 2023 Jul 7;9(27):eadh2019. doi: 10.1126/sciadv.adh2019. Epub 2023 Jul 7. PMID:37418517<ref>PMID:37418517</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8cli" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Eustermann S]]
[[Category: Girbig M]]
[[Category: Hauptmann L]]
[[Category: Hoffmann T]]
[[Category: Mueller CW]]
[[Category: Seifert-Davila W]]