View source for 8coz


==Structure of the catalytic domain of P. vivax Sub1 (triclinic crystal form)==
<StructureSection load='8coz' size='340' side='right'caption='[[8coz]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8coz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8COZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8COZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.438&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8coz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8coz OCA], [https://pdbe.org/8coz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8coz RCSB], [https://www.ebi.ac.uk/pdbsum/8coz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8coz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E6Y8B9_PLAVI E6Y8B9_PLAVI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The constant selection and propagation of multi-resistant Plasmodium sp. parasites require the identification of new antimalarial candidates involved in as-yet untargeted metabolic pathways. Subtilisin-like protease 1 (SUB1) belongs to a new generation of drug targets because it plays a crucial role during egress of the parasite from infected host cells at different stages of its life cycle. SUB1 is characterized by an unusual pro-region that tightly interacts with its cognate catalytic domain, thus precluding 3D structural analysis of enzyme-inhibitor complexes. In the present study, to overcome this limitation, stringent ionic conditions and controlled proteolysis of recombinant full-length P. vivax SUB1 were used to obtain crystals of an active and stable catalytic domain (PvS1(Cat)) without a pro-region. High-resolution 3D structures of PvS1(Cat), alone and in complex with an alpha-ketoamide substrate-derived inhibitor (MAM-117), showed that, as expected, the catalytic serine of SUB1 formed a covalent bond with the alpha-keto group of the inhibitor. A network of hydrogen bonds and hydrophobic interactions stabilized the complex, including at the P1' and P2' positions of the inhibitor, although P' residues are usually less important in defining the substrate specificity of subtilisins. Moreover, when associated with a substrate-derived peptidomimetic inhibitor, the catalytic groove of SUB1 underwent significant structural changes, particularly in its S4 pocket. These findings pave the way for future strategies for the design of optimized SUB1-specific inhibitors that may define a novel class of antimalarial candidates.

3D structures of the Plasmodium vivax subtilisin-like drug target SUB1 reveal conformational changes to accommodate a substrate-derived alpha-ketoamide inhibitor.,Martinez M, Batista FA, Maurel M, Bouillon A, Ortega Varga L, Wehenkel AM, Le Chevalier-Sontag L, Blondel A, Haouz A, Hernandez JF, Alzari PM, Barale JC Acta Crystallogr D Struct Biol. 2023 Aug 1;79(Pt 8):721-734. doi: , 10.1107/S2059798323004710. Epub 2023 Jul 10. PMID:37428845<ref>PMID:37428845</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8coz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Subtilisin 3D structures|Subtilisin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmodium vivax]]
[[Category: Alzari PM]]
[[Category: Barale JC]]
[[Category: Batista F]]
[[Category: Bouillon A]]
[[Category: Haouz A]]
[[Category: Martinez M]]