View source for 8cq2


==Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, C16S, C20S, C870S, T1279C mutant==
<StructureSection load='8cq2' size='340' side='right'caption='[[8cq2]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8cq2]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CQ2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cq2 OCA], [https://pdbe.org/8cq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cq2 RCSB], [https://www.ebi.ac.uk/pdbsum/8cq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cq2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9RN43_PHOLU Q9RN43_PHOLU] 
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== Publication Abstract from PubMed ==
Tc toxins are pore-forming virulence factors of many pathogenic bacteria. Following pH-induced conformational changes, they perforate the target membrane like a syringe to translocate toxic enzymes into a cell. Although this complex transformation has been structurally well studied, the reaction pathway and the resulting temporal evolution have remained elusive. We used an integrated biophysical approach to monitor prepore-to-pore transition and found a reaction time of ~30 hours for a complete transition. We show two asynchronous general steps of the process, shell opening and channel ejection, with the overall reaction pathway being a slow multistep process involving three intermediates. Liposomes, an increasingly high pH, or receptors facilitate shell opening, which is directly correlated with an increased rate of the prepore-to-pore transition. Channel ejection is a near-instantaneous process which occurs with a transition time of &lt;60 milliseconds. Understanding the mechanism of action of Tc toxins and unveiling modulators of the kinetics are key steps toward their application as biomedical devices or biopesticides.

Multistate kinetics of the syringe-like injection mechanism of Tc toxins.,Ng'ang'a PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Kuhnemuth R, Assafa TE, Dong M, Seidel CAM, Bordignon E, Raunser S Sci Adv. 2025 Jan 3;11(1):eadr2019. doi: 10.1126/sciadv.adr2019. Epub 2025 Jan 3. PMID:39752508<ref>PMID:39752508</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8cq2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photorhabdus luminescens]]
[[Category: Belyy A]]
[[Category: Nganga PN]]
[[Category: Prumbaum D]]
[[Category: Raunser S]]
[[Category: Roderer D]]