View source for 8d49


==Structure of Cas12a2 binary complex==
<StructureSection load='8d49' size='340' side='right'caption='[[8d49]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8d49]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfuricurvum_sp._PC08-66 Sulfuricurvum sp. PC08-66] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D49 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d49 OCA], [https://pdbe.org/8d49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d49 RCSB], [https://www.ebi.ac.uk/pdbsum/8d49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d49 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0C2W1L1_9BACT A0A0C2W1L1_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded DNA following recognition of a complementary RNA target, culminating in abortive infection(1). Here we report structures of Cas12a2 in binary, ternary and quaternary complexes to reveal a complete activation pathway. Our structures reveal that Cas12a2 is autoinhibited until binding a cognate RNA target, which exposes the RuvC active site within a large, positively charged cleft. Double-stranded DNA substrates are captured through duplex distortion and local melting, stabilized by pairs of 'aromatic clamp' residues that are crucial for double-stranded DNA degradation and in vivo immune system function. Our work provides a structural basis for this mechanism of abortive infection to achieve population-level immunity, which can be leveraged to create rational mutants that degrade a spectrum of collateral substrates.

RNA targeting unleashes indiscriminate nuclease activity of CRISPR-Cas12a2.,Bravo JPK, Hallmark T, Naegle B, Beisel CL, Jackson RN, Taylor DW Nature. 2023 Jan;613(7944):582-587. doi: 10.1038/s41586-022-05560-w. Epub 2023 , Jan 4. PMID:36599980<ref>PMID:36599980</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8d49" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfuricurvum sp. PC08-66]]
[[Category: Synthetic construct]]
[[Category: Bravo JPK]]
[[Category: Taylor DW]]