View source for 8d7f


==FlvF from Aspergillus flavus in complex with Bis-Tris==
<StructureSection load='8d7f' size='340' side='right'caption='[[8d7f]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8d7f]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_flavus_NRRL3357 Aspergillus flavus NRRL3357]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D7F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d7f OCA], [https://pdbe.org/8d7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d7f RCSB], [https://www.ebi.ac.uk/pdbsum/8d7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d7f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FLVF_ASPFN FLVF_ASPFN] Terpene cyclase; part of the gene cluster that mediates the biosynthesis of flavunoidine, an alkaloidal terpenoid with a tetracyclic cage-like core connected to dimethylcadaverine via a C-N bond and acylated with 5,5-dimethyl-L-pipecolate (PubMed:31885262). The tetracyclic core is synthesized by the terpene cyclase flvE and the cytochrome P450 monooxygenase flvD (PubMed:31885262). The terpene cyclase flvE catalyzes the cyclization of farnesyl pyrophosphate (FPP) to form (1R,4R,5S)-(+)-acoradiene and the cytochrome P450 monooxygenase flvD is then responsible for oxidative conversion of (1R,4R,5S)-(+)-acoradiene into the tetracyclic cage present in the final product flavunoidine (PubMed:31885262). The cytochrome monooxygenase flvC then hydroxylates the C-10 position (PubMed:31885262). The second terpene cyclase flvF is required for attachment of the C-7 axial dimethylcadaverine which is itself produced by the N-methyltransferase flvH and the decarboxylase flvG via methylation of L-lysine to give N,N-dimethyl-L-Lysine, and decarboxylation to afford dimethylcadaverine, respectively (PubMed:31885262). The NRPS flvI acylates the terpenoid core at the hydroxylated C-10 with dimethylpipecolate to yield final flavunoidine (PubMed:31885262). The bifunctional enzyme flvA and the dehydrogenase flvB are responsible for the synthesis of the dimethylpipecolate precursor (PubMed:31885262). The PLP-dependent lyase domain of flvA might use L-O-acetyl-homoserine and alpha-keto-isovalerate to form an intermediary ketone that can cyclize intramolecularly to yield an imine (PubMed:31885262). The imine can be reduced by flvB to yield the 6-carboxylated pipecolate (PubMed:31885262). The C-terminal alpha-KG-dependent oxygenase domain of flvA is then proposed to catalyze the decarboxylation to yield dimethylpipecolate (PubMed:31885262).<ref>PMID:31885262</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Aspergillus flavus NRRL3357]]
[[Category: Large Structures]]
[[Category: Christianson DW]]
[[Category: Tararina MA]]